Search results for " hollow fiber"
Article
Concentration and Diafiltration of Cell-Derived, Live Influenza Virus Using 750 C Hollow Fiber Filter Cartridge
Cross flow filtration (CFF) is a technique extensively used in vaccine production and particularly in influenza vaccine manufacturing. In contrast to normal flow (dead-end) filtration (NFF), the fee…
Article
Tools for Continuous Bioprocessing Development
These systems circulate cells between the conventional bioreactor and the hollow fibers to allow for more environmental control representative of large-scale production. The tangential flow geometry o…
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Efficient concentration of a low-titer bovine IgG with high recovery in low volume
Filtration was performed using ReadyToProcess™ hollow fiber filter cartridges. The described process enabled concentration of a bovine IgG antibody from 50.0 to 0.165 L in less than 5 h.
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New Therapies Present Scaling Challenges
New Therapies Present Scaling Challenges
Manufacturing differences between traditional mAb therapies and newer biotherapeutics dictate whether processes should be scaled up, scaled out, or use an al…
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Achieving Process Balance with Perfusion Bioreactors
AWS retains recirculating cells from a perfusion bioreactor without the need for a hollow fiber filter device, which is a widely used approach for cell retention. No membrane means no fouling or loss …
Article
Single-Use Workflow for Recovery of a Domain Antibody from E. coli Culture Feed in an Automated Manner
This application note demonstrates the performance of the automated ÄKTA™ readyflux single-use filtration system in microfiltration applications. Here, a domain antibody (dAb) expressed in E. col…
Article
Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
Culture support
Cell-detaching methods where direct access to the support is necessary, such as cell scraping, are not compatible with cell-culture supports such as microcarriers or hollow fibers.…
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
Before the sterile filtration step, the viral particles were concentrated and transferred into the final buffer by diafiltration using a ReadyToProcess hollow fiber cartridge (RTPUFP-500-C-9S). Finall…